The metIC operon involved in methionine biosynthesis in Bacillus subtilis is controlled by transcription antitermination

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The metIC operon involved in methionine biosynthesis in Bacillus subtilis is controlled by transcription antitermination.

There are two major pathways for methionine biosynthesis in micro-organisms. Little is known about these pathways in Bacillus subtilis. The authors assigned a function to the metI (formerly yjcI) and metC (formerly yjcJ) genes of B. subtilis by complementing Escherichia coli metB and metC mutants, analysing the phenotype of B. subtilis metI and metC mutants, and carrying out enzyme activity ass...

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Title The metIC operon involved in methionine biosynthesis in Bacillus subtilis is controlled by transcription antitermination

The metIC operon involved in methionine biosynthesis in Bacillus subtilis is controlled by transcription antitermination The metIC operon involved in methionine biosynthesis in Bacillus subtilis is controlled by transcription antitermination There are two major pathways for methionine biosynthesis in microorganisms. Little is known about these pathways in Bacillus subtilis. The authors assigned...

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Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.

The characterization of a three-gene operon (the thiC operon) at 331 min, which is involved in thiamine biosynthesis in Bacillus subtilis, is described. The first gene in the operon is homologous to transcription activators in the lysR family. The second and third genes (thiK and thiC) have been subcloned and overexpressed in Escherichia coli. ThiK (30 kDa) catalyzes the phosphorylation of 4-me...

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Transcription of the nfrA-ywcH operon from Bacillus subtilis is specifically induced in response to heat.

The NfrA protein, an oxidoreductase from the soil bacterium Bacillus subtilis, is synthesized during the stationary phase and in response to heat. Analysis of promoter mutants revealed that the nfrA gene belongs to the class III heat shock genes in B. subtilis. An approximate 10-fold induction at both the transcriptional and the translational levels was found after thermal upshock. This inducti...

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Kinetic analysis of tRNA-directed transcription antitermination of the Bacillus subtilis glyQS gene in vitro.

Binding of uncharged tRNA to the nascent transcript promotes readthrough of a leader region transcription termination signal in genes regulated by the T box transcription antitermination mechanism. Each gene in the T box family responds independently to its cognate tRNA, with specificity determined by base pairing of the tRNA to the leader at the anticodon and acceptor ends of the tRNA. tRNA bi...

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ژورنال

عنوان ژورنال: Microbiology

سال: 2002

ISSN: 1350-0872,1465-2080

DOI: 10.1099/00221287-148-2-507